diff --git a/numba/cuda/cuda_paths.py b/numba/cuda/cuda_paths.py
index 0da435d33..7b1fde087 100644
--- a/numba/cuda/cuda_paths.py
+++ b/numba/cuda/cuda_paths.py
@@ -24,10 +24,7 @@ def _find_valid_path(options):
 
 def _get_libdevice_path_decision():
     options = [
-        ('Conda environment', get_conda_ctk()),
-        ('CUDA_HOME', get_cuda_home('nvvm', 'libdevice')),
-        ('System', get_system_ctk('nvvm', 'libdevice')),
-        ('Debian package', get_debian_pkg_libdevice()),
+        ('Nix store', get_nix_ctk('nvvm', 'libdevice')),
     ]
     by, libdir = _find_valid_path(options)
     return by, libdir
@@ -35,16 +32,14 @@ def _get_libdevice_path_decision():
 
 def _nvvm_lib_dir():
     if IS_WIN32:
-        return 'nvvm', 'bin'
+        return 'bin',
     else:
-        return 'nvvm', 'lib64'
+        return 'lib64',
 
 
 def _get_nvvm_path_decision():
     options = [
-        ('Conda environment', get_conda_ctk()),
-        ('CUDA_HOME', get_cuda_home(*_nvvm_lib_dir())),
-        ('System', get_system_ctk(*_nvvm_lib_dir())),
+        ('Nix store', get_nix_ctk(*_nvvm_lib_dir())),
     ]
     by, path = _find_valid_path(options)
     return by, path
@@ -64,14 +59,12 @@ def _cudalib_path():
     if IS_WIN32:
         return 'bin'
     else:
-        return 'lib64'
+        return 'lib'
 
 
 def _get_cudalib_dir_path_decision():
     options = [
-        ('Conda environment', get_conda_ctk()),
-        ('CUDA_HOME', get_cuda_home(_cudalib_path())),
-        ('System', get_system_ctk(_cudalib_path())),
+        ('Nix store', get_nix_lib_ctk(_cudalib_path())),
     ]
     by, libdir = _find_valid_path(options)
     return by, libdir
@@ -82,6 +75,22 @@ def _get_cudalib_dir():
     return _env_path_tuple(by, libdir)
 
 
+def get_nix_ctk(*subdirs):
+    """Return path to nix store cudatoolkit; or, None if it doesn't exist.
+    """
+    base = '@cuda_toolkit_path@'
+    if os.path.exists(base):
+        return os.path.join(base, *subdirs)
+
+
+def get_nix_lib_ctk(*subdirs):
+    """Return path to nix store cudatoolkit-lib; or, None if it doesn't exist.
+    """
+    base = '@cuda_toolkit_lib_path@'
+    if os.path.exists(base):
+        return os.path.join(base, *subdirs)
+
+
 def get_system_ctk(*subdirs):
     """Return path to system-wide cudatoolkit; or, None if it doesn't exist.
     """